Babiichuk L. Effect of cryopreserved cord blood on structural and functional state of cardiovascular system in animals with experimental hypertension (experimental study).

The research object was the structural and functional state of cardiovascular system of animals of various age groups in the norm, under conditions of stress-induced hypertension and after the introduction of cryopreserved cord blood nucleated cells (cCBNCs). The research aim was to evaluate the effect of cryopreserved human cord blood nucleated cells on the structural and functional state of cardiovascular system of animals of different age groups in the model of stress-induced arterial hypertension. Research methods were as follows: cryobiological, cytometric, cytological, electrophysiological (heart rate variability analysis), electron microscopy, histochemical, biochemical, immunoassay, stress-induced hypertension simulation and statistical analysis of the data. For the first time, it has been found, that the cryopreservation of CBNCs, including stem hemopoietic cells, under the protection of DMSO as a cryoprotectant at 5% concentration, after isolation in polyglyucine, allows to preserve more than 80% of the CD45+ cells and more than 90% of CD34+ cells in a viable state. It has been shown that after introduction to with arterial hypertension of cCBNCs, the autonomic nervous system and humoral link of regulation were activated, the trophy of the myocardium tissues and vessels was restored, the lipid homeostasis was normalized, the level of thyroid hormones and nitrites in blood serum and the indices of blood pressure were normalized.