Odnosum H. Nosema disease of honey bees (distribution and prevention methods)

The dissertation thesis summarizes the results of investigation of the distribution of Nosema ceranae and Nosema apis ‒ obligate intracellular parasites of the honey bees in the apiaries in Ukraine, scientifically based рrevention methods. It was established that Nosema ceranae is present in most of the analyzed samples of bees, selected from bee families affected by Nosema disease, and prevails over Nosema apis in the most regions, which belong to different natural climatic Ukrainian zones. A comparative analysis of the influence of doses of the invasion of spores Nosema apis and Nosema ceranae (5×102‒5×107 spores per bee) on the dynamics of their extinction was conducted. Influence of biologically active substances of animal origin in different concentrations (0.1 %; 0.5; 1 %) (homogenate of the waxworm Galleria mellonella L. and drone honey bee larvae Apis mellifera L.) and plant origin (complex plant preparation KAS-81 (0.35 %) and its components ‒ pine bud extract Pinus sylvestris L. (0.0175 %) and the wormwood Artemisia absinthium L. (0.3325 %), compared to the most common antibiotics against Nosema spp. (Nosemat and Fumagilin-B, dosage according to the manufacturer's instructions), on the dynamics of bee extinction at Nosema disease was investigated in laboratory conditions. The efficiency of the investigated substances has been verified on bee families with a natural invasive background.