Pushkareva T. Changes in the structure of stromal microenvironment, bone marrow hematopoiesis and prediction of the course of acute leukemia in children

The purpose of the study was to determine the criteria for predicting the course of GF in children based on the evaluation of changes in the morphofunctional state of hematopoiesis, bone tissue and stromal microinjection in the stages of chemotherapy. The objective of the study was GLL, GML, peripheral blood and bone marrow, culture of stromal fibroblasts, urine of patients.In the first studied of collagen formation, starting with the colony formation of stromal fibroblasts of the bone marrow in patients with acute leukemia, and is indicated for the the suppression of these processes. The high proliferative colony formation potential (higher than 60.0) and below 11.0 for 105 nuclei cells correlated with shorter survival of the patients.The inverse correlation between the indicator of densitometry and the excretion of dihydrophosphates and dihydrophosphates of calcium tetrahydrates in urine, and alkaline phosphatase activity was established. The densitometric index is below 65 units correlated with short survival of patients (up to 30 months).New data were obtained for restoration of bone marrow in patients with acute lymphoblastic leukemia after myelosuppressive therapy. The increase of erythroid elements higher, than the standard at the first stage of chemotherapy, correlates with the shorter survival of children, as compared with the restoration of the granulocyte-monocytic type, that may be an additional criterion for predicting the course of the disease.