Povar M. Systemic and organ-specific pathobiochemical changes in rats with streptozotocin-induced diabetes mellitus, complicated by incomplete global ischemia-reperfusion of the brain

Object - ischemic-reperfusion damage of the brain in streptozotocin-induced diabetes mice; the goal is to study the dynamics of cerebral, organ-specific and systemic effects of incomplete global ischemia-reperfusion of the brain in streptotrozine-induced diabetes mice according to pathobiochemical criteria; methods - pathophysiological; biochemical; microbiological; mathematical; results - The thesis presents the results of studying the dynamics of cerebral ischemia-reperfusion effect on pathobiochemical disorders in the brain, liver, spleen, blood, and on the species and population composition of the colon microbiota in rats with streptozotocin-induced diabetes mellitus (DM); DM was first demonstrated to change the character of pathobiochemical disorders induced by ischemia-reperfusion not only in the structures of neocortex and hippocampus, but in the liver, spleen and blood as well. In the structures of the brain and blood DM restricts the signs of oxidative stress associated with ischemia-reperfusion, especially at the late post-ischemic period, and eliminates activation of proteolytic activity peculiar for animals without diabetes, promotes formation of cerebral hypocollagenosis and hypofibrinolysis during the whole period of observation; removes the reaction of fibrinolytic activity parameters in the liver and spleen at the late post-ischemic period characteristic for the control animals, restricts the reaction of proteolytic systems of these organs on the 12th day. DM was found to modify reaction of protease-antiprotease system in the blood on cerebral ischemia-reperfusion during both terms of post-ischemic period. In rats without DM at the early ischemic-reperfusion period intensification of pro-coagulant potential is partially balanced by the activation of anti-coagulant mechanisms and fibrinolysis, and till the 12th day the parameters of hemostasis system become normal. In rats with diabetes the factor intensifying thrombotic risk remain activated during the whole term of observation against the ground of inhibition of the blood fibrinolytic activity which deteriorates conditions of thrombolysis. DM complications by cerebral ischemia-reperfusion intensify the signs of dysbiosis in the general specimen of the colon peculiar for diabetes and cerebral ischemia-reperfusion in separated simulation.