Korol-Bezpala L. The improvement of biotechnology of Chironomus larvae cultivation and use in fisheries.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0420U000033

Applicant for

Specialization

  • 03.00.20 - Біотехнологія

06-02-2020

Specialized Academic Board

Д 27.821.01

Essay

In the dissertation on the basis of experimental data the technology of cultivation of biomass of Chironomus larvae has been improved. The efficiency of its use for growing small carp has been proved. In order to grow safe from infectious diseases biomass of Chironomuslarvae the effect of different conditions of pasterurization on the content of microorganisms in the sludge was studied. It was experimentally found that the smallest number of microorganisms was isolated in the crops of pasteurized sludge with instant pasteurization (95° C), the number of bacteria was 1,3 ? 106 CFU/1g. During the prolonged pasteurization (65° C), the number of colony-forming organisms decreased by 13 times, during short-term pasteurization (75° C) - by 18 times, and in case of instant pasteurization (95° C), the number of colony-forming organisms decreased by 285. It has been established that in the silt of the Ros River basin as a major component of the nutriculture medium for Chironomus larvae, microorganisms are mainly represented by colonies of Pseudomonas spp. - 45,9 %, Bacillus sp. - 32,4 % and coccalmicroflora - 21,7 %. It has been proved that yeasting of sunflower meal increases in the latter the lysine content - by 29,3 %, phenylalanine - by 26,6, histidine - by 5,6, leucine and isoleucine - by 24,6, methionine - by 57,2, valine - by 41,8, proline - by 23,3, threonine - by 77,6, serine - by 34,1, alanine - by 46,8 and glycine - by 36,4 % in relation to its native form.

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