Kharytonenko N. Variability of Vitamin E (Tocopherol Isomers) Content and Inheritance Patterns in Sunflower Lines and Inbred Generations

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0420U101072

Applicant for

Specialization

  • 06.01.05 - Селекція і насінництво

07-07-2020

Specialized Academic Board

Д 64.366.01

Plant Production Institute nd. a. V. Ya. Yuriev

Essay

Object: redistribution of tocopherol by increasing β-, γ- or δ-isomer contents in sunflower seeds in breeding for quality. Objective: to determine the variability of tocopherol isomers in starting material, lines, and inbred generations of sunflower and to create new lines - pollen fertility restorers with altered tocopherol profile, which are adapted to the growing conditions of the Eastern Forest-Steppe of Ukraine. Methods: general scientific, field, special, laboratory, mathematical and statistical. Novelty is in solving an important scientific challenge to establish the content variability and inheritance patterns of vitamin E (tocopherol isomers) in sunflower lines, mutants and inbred generations. For the first time in Ukraine, the variability of the contents of different tocopherol isomers and their sum was studied. Based on this, starting material and new lines were categorized into classes according to the contents of different tocopherol isomers and their sum. The peculiarities of new breeding material – inbred lines with altered profile of tocopherol isomers in combination with other valuable economic features are described. The inheritance peculiarities in terms of dominance degree and heterosis effect for the tocopherol isomer contents were established. For the first time in Ukraine, new lines - pollen fertility restorers with altered contents of tocopherol isomers in combination with valuable economic features were developed, which enriched breeding programs with new material to create sunflower hybrids in future. Results: Based on the 2013-2018 study results, 10 lines - pollen fertility restorers with altered contents of tocopherol isomers in combination with other valuable economic features (Kh177V, Kh1711V, Kh1712V, Kh1716V, Kh1717V, Kh1719V, Kh1725V, Kh1738V, and Kh1747V) have been selected and registered with the NCPGRU. The lines enriched the bank of plant genetic resources. The catalog of starting material for sunflower breeding for quality was compiled. The catalog contains characteristics of the 10 lines - pollen fertility restorers with altered profile of tocopherol isomers in combination with other valuable economic features. Degree of Implementation: The developed lines - pollen fertility restorers with altered contents of tocopherol isomers have been implemented in breeding and research programs of the Plant Production Institute named after VYa Yuriev of NAAS of Ukraine. Scope of Implementation: scientific institutions of NAAS, NAS and the Ministry of Agrarian Policy and Food of Ukraine, educational establishments, agrarian enterprises of Ukraine.

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