The object of research is the process of professional preparation of future specialists in physical culture and sports. The purpose of the study is to substantiate theoretically, develop and test the pedagogical conditions of development of kinesiological competence of future specialists in physical culture and sports on the basis of a differentiated approach experimentally. The scientific novelty of the obtained results is that: for the first time the pedagogical conditions of development of kinesiological competence of future specialists in physical culture and sports on the basis of a differentiated approach: 1) stimulating motivation for the development of motor skills through the organization of systematic open monitoring of individual achievements of each; 2) combination of individual character of assimilation of kinesiological knowledge on the collective organization of educational activity of students through differentiation of the maintenance of training; 3) integration of innovative types of motor activity and variable didactic materials through individualization of the educational and training process were identified and substantiated scientifically; the model of development of kinesiological competence of future specialists in physical culture and sports on the basis of a differentiated approach was developed, substantiated theoretically and tested experimentally. This model based on a set of general didactic and specific principles, based on certain pedagogical conditions and provides for improving the content of training through the use of forms, methods and means that take into account biological, gender, social, anatomical, physiological, physical and psychological characteristics of the individual and group. The essential characteristics of the concepts "kinesiological competence of future specialists of physical culture and sports", "differentiated approach in the development of kinesiological competence of future specialists of FC&S", "development of kinesiological competence of future specialists of physical culture and sports" are specified. The structure of kinesiological competence of future specialists in physical culture and sports in the unity of motivational, activity-cognitive and reflexive components is described. Diagnostic tools for assessing the levels (low, medium, high) of the development of kinesiological competence of future specialists in physical culture and sports, which contains criteria (need, practical-subject, behavioral), indicators (the need for development of kinesiological competence, the amount of kinesiological knowledge, level of kinesiological skills, methodological skills of kinesiological skills, reflection) are developed with an appropriate set of diagnostic techniques. The content, forms and methods of professional training of future specialists in physical culture and sports aimed at the development of kinesiological competence on the basis of a differentiated approach have been improved.