Liubchenko I. Creation of source material of camelina sativa resistant to stress factors with the use of biotechnological methods

The dissertation is devoted to the decision of a problem of perfection of technology of creation of a source material of camelina sativa resistant to salinity and osmotic stress for use in the technological scheme of cellular selection. The technology of obtaining a sterile culture of camelina sativa has been developed: the conditions for sterilization of explants have been selected, the nutrient medium for induction, passage and morphogenesis of callus tissue and microclonal propagation of plant material has been modified. The stress effect of sodium chloride and mannitol on the callus tissue of camelina sativa was established and the optimal concentrations of selective factors for in vitro selection were selected. Multistage cell selection for resistance to sodium chloride was developed and performed, which allowed to obtain a source material of camelina sativa resistant to 1.5% salinity. The possibility of preserving the trait of biomaterial stability during the transition from cellular to intact plant level is proved and the heredity of resistance to stress factor is shown, which confirms the genetic nature of changes that occur during in vitro selection. The complex resistance of salt-resistant plant lines to osmotic stress has been established. The evaluation of stable somaclonal lines obtained by cell selection methods in ex vitro conditions on the complex of biological and economically valuable features is carried out. Selection samples (C-87-7, C-121-2, П-46-5 and П-646-3) with high individual seed productivity, which can be used as a source material to create high-yielding varieties of camelina sativa resistant to adverse environmental factors.