Mirzebasov M. Pathogenesis of the duodenal wall injuries under the action of volatile compounds of epoxy resins

The goal of the dissertation work was to determine the pathogenesis of the rat duodenum damages as a result of epichlorohydrin long-term exposure based on the evaluation of duodenum morphofunctional status and to determine the efficacy of Echinacea purpurea extract and thiotriazoline use in adjustment of the disorders occurred. In the study it was shown that epichlorohydrin long-term exposure leads to the changes in condition of the rat duodenum including a decrease in the thickness of duodenum wall, duodenum mucosa, duodenum muscle membrane and an increase in the thickness of duodenum submucous tissue and serous membrane as well as the development of duodenum mucosa hypotrophy, which consists in a decrease in villus height and crypt depth. Epichlorohydrin exposure causes disturbances in the cellular composition of the villus and crypts epithelium in the rat duodenum, a decrease in mitotic activity of epithelium cells in duodenum mucosa, the changes in condition of epithelium columnar cells in the villi and crypts, disturbance of the villus and crypts mitochondria in columnar epitheliocytes of duodenum mucous coat. The mechanism of epichlorohydrin action involves disruption of mitochondria state that affected negatively the energy supply for the mitosis division processes. As a result, cell renewal processes were disturbed, the cell number in the structural components of duodenum mucosa was reduced, duodenum mucosa hypotrophy occurred, and cell differentiation processes were deteriorated. The use of Echinacea purpurea extract in the setting of epichlorohydrin exposure reduced the severity and duration of epichlorohydrin negative effects. Morphofunctional state of the duodenum wall, cellular composition and epithelial cells state in the duodenum mucosa were improved, and expressiveness of the duodenum mucosa hypotrophy was reduced. Echinacea purpurea extract reduced the duration of mitotic activity inhibition induced by epichlorohydrin and duration of area of one mitochondria of epithelium columnar cells reduction in the crypts of duodenum mucosa. The use of thiotriazoline concomitantly with epichlorohydrin exposure mitigated the damaging effects of epichlorohydrin. It is manifested in improvement of morphofunctional state of duodenum mucosa, in reducing a severity of the duodenum mucosa hypotrophy, in improvement of cellular composition of villi and crypts epithelium in the duodenum mucosa, in reducing a period of mitotic activity inhibition, in reducing a period of area of one mitochondria and area of mitochondria in 100 μm2 cytoplasm reduction of epithelium columnar cells in the crypts.