The dissertation is devoted to the clarification of the peculiarities of changes in
the functional state of the mesenteric lymph nodes in the rats with experimental
gestational diabetes and in the induction of oral insulin tolerance by means of
immunofluorescence and molecular genetic methods.
It has been established that in the offspring of experimental rats with gestational
diabetes, a significant decrease in the content of mRNA of the autoimmune regulator
Aire was 8.1 times in the 1-month and 2.3-fold in the 6-month rats. The content of
mRNA of the transcriptional regulator Deaf1 in 1-month-old animals did not
significantly change, and in the 6-month-old descendants it was observed to decrease
by 9.2 times. Regarding mRNA of the transcription factor Foxp3, in the 1-month-old
rats, a significant decrease was detected – 50-fold, and 6-month – two-fold, compared
to control. Animal groups, the descendants of rats with EGD, which were injected
insulin over the first 14 days of life, showed the following results: in the 1-month-old
rats, transcriptional induction of the Aire gene was observed: the content of the
autoimmune regulator mRNA increased by 13.2 times, at 6 months at 2 times The
transcriptional regulator Deaf1 in the first age group showed a significant increase –
11.5 times. The Foxp3 transcription factor expression study showed that in one-monthold
rats, the content of its mRNA increased by 5.2-fold, and in the 6-month-old – by
3.3-fold.
In the experimental groups receiving oral insulin, the expression of the mRNA
of the costimulatory molecules Ctla4 and the Treg-dependent suppressor cytokine IL-
8
10 was also studied. The following results were obtained: at the age of 1 month, the
relative number of mRNA of the Ctla4 gene increased by 12.2 times, and at the age of
6 months, it did not change significantly. The IL-10 mRNA content, on the contrary,
did not change in the first age group, but in the 6-month-old rats it increased 15-fold.
It has been shown that in the offspring of rats with EGD there is a significant
increase in the content of the mRNA of the gene Ccr7 – 26.8 times in 1-month and 21
times in the 6-month-old descendants. Regarding the content of the mRNA receptor
Cxcr4, in both age groups it has not significantly changed. Relative normalized amount
of mRNA addresses MAdCAM-1 at the age of 1 month increased by 2.4 times, and in
6 months – by 2.3 times. In the study of expression of the S1pr1 gene, the following
results were obtained: in the first age group, the S1PR1 mRNA content increased 3.6-
fold; in older animals, this figure increased 5-fold. Animal groups, the descendants of
experimental gestational diabetes mice who were given oral insulin during the first 14
days of life showed the following results: the amount of mRNA of the Ccr7 gene in 1-
month-old animals decreased by 81 %, and no significant changes were observed in 6-
month-old rats. Expression of the gene Cxcr4 has not undergone a significant change
in both age groups. The number of mRNA addresses MAdCAM-1 at the age of 1 month
decreased by 95 %, while in the 6-month rats, the increase was 3.9-fold. The content
of the mRNA of the S1pr1 gene in rats in the first age group decreased by 65 % and
the older ones by 96 %.
