Koval O. The development of innovative technology of bioethanol from sugar beet processing products

Thesis is devoted to the experimental substantiation of the concept of bioethanol production integration into the technological process of sugar beet processing into sugar, which allows to regulate the ratio of sugar: bioethanol commodity production depending on demand. In the process of experimental work performing research methods generally accepted in the alcohol and sugar industry were used. The dependence of the formation and yield of bioethanol from sugar intermediates on their chemical composition and biochemical properties was theoretically and experimentally confirmed The basic kinetic regularities and rational technological parameters of sugar intermediates` fermentation in bioethanol were established. Scientific provisions on the influence of the colloid-chemical composition of the non-sugar part of sugar beet processing products on the enzymatic activity of yeasts and the biosynthesis of ethyl alcohol were further developed. Based on theoretical and experimental research the influence of coloring agents on biochemical property of yeasts and necessary degree of rationalization of the technological scheme of processing of sugar beet processing products into bioethanol was substantiated. It was established that non-sugars of concentrated sugar beet processing products, in particular the outflow of the second crystallization massecuite and molasses, have a pronounced negative effect on the growth rate of yeast biomass and their alcohol-forming ability. The effect of antiseptics on the synthesizing ability of the yeasts Saccharomyces cerevisiae was studied. The expediency and ecological efficiency of oxalic acid use as an acidifying agent of the fermenting technological medium instead of mineral acids was proved. The expediency of diffusion juice fermentation in a mixture with molasses or the outflow of the second crystallization massecuite for increase the alcohol content in mature wort to 11.5 - 11.6% vol. was experimentally substantiated. Technological parameters that affect the efficiency of alcohol fermentation were established and experimentally confirmed. Technological regulations for bioconversion of sugar-containing raw materials into bioethanol were developed for PJSC Gnidavsky Sugar Plant. According to the developed technological regulations, the production of bioethanol at the facilities of Bio PEK LLC (Lutsk) in the conditions of the sugar plant (PJSC Gnidavsky Sugar Plant) was carried out. The daily plant capacity is 6,000 decaliters per day. Technological modes of continuous fermentation of sugar beet processing products by immobilized cells were experimentally confirmed, which provides fermentation rate, sugar economy on yeasts cultivation, reduction of fermentation equipment metal capacity and fermentation time. Experimental technological regulations for obtaining molasses-alcohol mature wort by immobilized yeasts cells were developed. It was determined that under the conditions of removal of different types of sugar beet processing products from sugar production and fermentation of wort based on them into bioethanol, the increase in enterprise income may be 2.3 - 23.1%.