In the dissertation work for the first time on the basis of complex researches the comparative analysis of influence of a diabetes mellitus on degree of activity of free radical oxidation, reactivity of antioxidant system, level of markers of inflammation, microbial contamination and nonspecific immunity, and also reparative ability of dermal tissues.
In experimental diabetes mellitus, it was found that after using skin glue, the thickness of the skin epidermis of rats on day 28 of the wound process reliably does not differ from the intact group, while after using surgical sutures, its thickness is reliably smaller. It was found that the rates of free radical oxidation in the skin homogenate of rats with induced diabetes mellitus after using skin glue were significantly higher than in healthy animals during the wound process in all days of observation, but significantly lower values of primary products of lipid peroxidation in skin homogenate the use of skin glue in relation to surgical sutures. The intensity of protein oxidative modification in diabetic rats after using surgical sutures in the wound process was reliably higher in all days of observation compared to data after using skin glue, while the level of nitrogen monoxide metabolites did not depend on the method of wound closure. It was shown that in animals with experimental diabetes mellitus, depending of the type of wound closure, the activity of enzymatic and non-enzymatic antioxidant compounds was higher than intact animals at all days of observation, but in animals treated after use of skin glue, the values were significantly lower, in relation to group data after using surgical sutures. It is proved that the wound process in experimental diabetes mellitus in rats leads to reliable contamination of the wound with pathogenic and opportunistic microflora and a decrease in nonspecific immunity in all observation periods, in relation to intact animals. But, in the remote term of research in the skin homogenate of diabetic rats after using skin glue, the level of urease is lower, and the level of lysozyme is higher by 7.0 % than of rats with diabetes, after use of surgical sutures. It is proved that the wound process in experimental diabetes mellitus in rats leads to reliable contamination of the wound with pathogenic and opportunistic microflora and a decrease in nonspecific immunity in all observation periods, in relation to intact animals, but in the long term in the skin homogenate of diabetic rats skin glue, the level of urease is lower, and the level of lysozyme is 7.0 % higher than that of rats with diabetes, which used surgical sutures. It has been shown that in experimental diabetes, the reparative capacity of the skin due to the wound process is reduced in relation to healthy.
