ANDRIYUK L. Clinicopathologic substantiation of the use of factors of nucleic nature for diagnosis, treatment and prevention of vascular diseases of the brain.

Pathogenic significance of the condition of parameters of nucleic homeostasis in chronic and acute processes of cerebrovascular diseases of ishemic nature was pointed in the dissertation based on the clinicoinstrumental, clinicobiocamical and experimental investigations. The changes of data of nucleic homeostasis in blood and cerebrospinal fluid in the patients with different forms of chronic and acute ishemic lesions of the brain were researched. It was revealed that changes of the condition of the nucleic homeostasis depends on the acuity of the disease, mechanisms of the pathophysiologic processes, severity of the course and the location of vascular dysgemia. In the case of ishemic stroke it was detected the decreased level of DNA, RNA and total nucleic acids in the area of necrosis and increased activity of deoxyribonuclease I and ribonuclease II. In the marginal region of infarction the decreased level of nucleic acids is two times lower. Also, there is increased activity of ribonuclease II and deoxyribonuclease II. Ishemia produces the damage of the brain tissue which results in the lesion of the nucleic homeostasis and pathogenicaly corresponds the changes of nucleic data in the blood and liquid. That's why the phenomenon of "blow out" consists in the decreased level of DNA and RNA in the area of necrosis and penumbra and correspondingly their increase in the blood serum and cerebrospinal fluid. The initiated complex therapy with the use of ribonucleic acid in patients with chronic and acute disorders of cerebral blood flow of the ishemic nature provides considerably positive impact on the effectiveness of the treatment, which is confirmed by the dynamic of the main clinical manifestations, dopplersonography findings, data of nucleic acids and activity of nucleases in the blood serum and liquid.