Object of research: physiology-biochemical processes in ejakulate and spermatozoa of bulls, follicles of ovaries, oocytes and embryos of cows. Purpose of research: Тo learn the role of oxidizing processes in ejakulate of bulls and follicles of ovaries of cows, their influence on capacity and ripening of spermatozoa and oocytes out of organism, to develop the methods of correction of metabolism of gametes, evaluation of them, the physiology, morphological state and fitness for the use in practice of fertilization and reproductive biotechnology, increase of fertilization capabilities of spermatozoa, impregnated of oocytes of in vivo and in vitro. Methods of research and equipment: physiologycal, biochemical, citomorfological, biotechnological, statistical; microscope, Goryaev apparatus, calorimeter, spectrumphotometr, electrophoresis devise, potentiometer, polarograph. The theoretical and practical results: The role of redox processes is grounded for providing of physiology descriptions of spermatozoa, capacitacion and impregnating ability, in oocytes - influence on ripening in the follicles of ovaries and out of organism, impregnated and development of embryos. Results of researches are theoretical subsoil for development of new effective environments for cryoconservation sperm of bulls and cultivation of embryos of cows, methods of upgrading ejakulate and impregnating ability of spermatozoa, survival and development of embryos. The practical value of work consists in development of methods of increase of efficiency impregnation of cows and reproductive biotechnology, providing of speed-up reproduction of highly productive and maintainance of gene pool of rare animals. The novelty: The role of redox processes is first generalized in forming and providing of physiology indexes of quality and fertilization of spermatozoa of bulls of in vivo and in vitro, ripening of oocytes, their impregnation and development of embryos of cows of in vitro. First scientific task was fulfilled by the experimental investigationof flow and regulation of redox processes at ripening and capacitacion spermatozoa in vitro and oocytes – in the process of ripening and after the impregnation – growth of embryos. First found factors which provide optimum correlation of redox processes and renewal in gametes, the markers of intensity of the indicated processes are set. The stage of innovation: Methods from the objective and reliable evaluation of quality of gametes of bulls and cows, correction of intensity of redox processes in spermatozoa and oocytes, increases of fertilization of spermatozoa and impregnation of cows and heifers in vivo and oocytes – in vitro were developed. Recommendations and SOU from the evaluation of quality of ejakulate bulls after activity of oxidizing enzymes, method of increase of viability, survival and impregnating ability of spermatozoa, in the process of preparation of sperm to freezing, method of growing of embryos of cows in vitroare developed. Sphere (field of using): physiology of man and animals, artificial impregnation, reproductive biotechnology.
