Limanskaya O. Molecular technologies for typing and detection of bacterial and viral pathogens

Object: genomic DNA of Mycobacterium tuberculosis complex, members of Bacillus cereus sensu lato group, Proteus sp., proviral DNA of bovine immunodeficiency virus and bovine leukemia virus. Goals: creation of reliable technologies of early detection and genotyping of Mycobacterium tuberculosis complex, Proteus sp., retroviruses and other socially significant pathogens and development of approaches for structural differentiation of genomes of human and animal pathogens. Methods: computer analysis, conventional polymerase chain reaction and its modifications with following restriction fragments length polymorphism analysis and sequencing amplicon, atomic force microscopy, UV-spectroscopy with thermal denaturation, matrix-assisted laser desorption ionization mass spectrometry. Results: molecular genetic sets for differentiation of closely related mycobacteria tuberculosis complex, widespread pathogens of human urogenital tract Proteus mirabilis and P. vulgaris, and species-specific detection of members of Bacillus сereus sensu lato group have been designed. Technology for detection of single nucleotide polymorphisms accosiated with resistance of mycobacteria tuberculosis to antituberculosis drugs has been developed. Novelty: new decision for the problem of enhancement of efficiency for methods of molecular detection and typing of of socially significant bacterial and viral pathogens and search of structure-functional markers in their genome has been found. Introduction: technology for detecting of point mutations in the genome of the pathogens can be exploited for determination of genetically deterministic resistance to drugs for causative agents of infectious diseases. Employment: developed methods for detection of genomic nucleic acids of socially significant human and animal pathogens can be applied for carrying out of epidemiological, epizootic and diagnostical studies in the health and veterinary medicine organizations.