Merzlov S. Theoretical and practical base for developing mobilisation biotechnologies of biologically active complexes and their applying in poultry farming

The dissertation is devoted deals with development of biotechnologies of ferments immobilisation: Ladosym fitase, amilosubtyline, protosubtyline, Iodine and organic form of Cobalt to increase biologically active complexes efficiency increase and antinutritional factor of cereals fitate decrease in poultry mixed fodder.We have developed the technology of metalochelate immobilization of Ladozym fitase proxy on the domestic natural mineral saponite modified with Ferum (III) Chloride under Ca2+ participation considering the explored structure, active centre biocatalysator charge and the effector action.The adsorbtion way of immobilization of amylosubtyline and protosubtyline was worked out on the saponite of Khmelnytsk oblast deposit and on ceolitecontaining basalt tuff of Polytsk II deposit of Rivne oblast.It has been experimentally proved that saponite is the optimal carrierfor amylosubtyline, and ceolitecontaining basalt tuff is a carrier for protosubtyline.The way of Iodine immobilization on natural mineral saponite by absorption was de-veloped. The immobilized on saponite Iodine is not eliminated into the environment for two months in premixes. Carrier absorbed Iodine is extracted into the environment whose pH imitates the reaction of monogastric animals.The technology of production of mixedligand Cobald complexes under the reaction of coproportionality MeA2+MeL2=2MeAL, where Me stands for metal, A and L stand for complexforming aminoacids. It has been proved in the experiments that maximum concen-tration of the element in complexes can be achieved under the following correlation metal:lysine:glicine - 1:1:1. Toxicologic experiments in white mice, rats and quails with applying physiologic bio-chemical, histological and pathology anatomic research results proved that DL50 for mix-edligand Cobalt complex is correspondently 4,08 g/kg; 1,55 and 3,4 g/kg weight. The mixedligand Cobalt complex belongs to the III class according to their acute and chronic toxity. They are moderate harmful, do not cause irritation of mucous membrane in the laboratory animals' eyes and gas low cumulative properties. Under constant study of injecting immobilized Iodine to mice do not cause the death. Immobilized Iodine can be referred to low harmful according to the research.It has been proved that applying immunoferment fitase combined with immobilized Iodine, amilosubtyline, protosubtyline, and Cobalt mixedligand complex provides in-crease in fitates hydrolease, Phosphorus uptake from mixed fodder and increase in live weight in poultry which is profitable: mixed fodder consumption per production unit decrease by 3,1-6,5%.