Voznesenska T. Reproductive system organs functioning in conditions of experimental immune ovarian failure

The dissertation is devoted to research of functioning of organs of reproductive and immune systems in conditions of experimental immune ovarian failure. It has been established, that proinflammatory cytokines inhibit oocyte meiotic maturation, carry carry damaging effects on cumulus cells, while the nature and degree of damage depended on the type of cytokine, reduces contractile activity of myometrium. It is shown that the development of oocytes and contractile activity of the myometrium are determined by the functional state of mitochondria. It is received the new data on the possible reason of occurrence and morphological features of mitochondrion dysfunctions in murine oocytes and cumulus cells. It is proved, that immunization of animals by allogeneic ovarians can be used as model of experimental immune ovarian failure. It is established, that such immunization of animals results in damage of ovarians at animals, to changes the contractile activity of the myometrium and oviduct, increased mortality of embryos and delay their development. It is revealed, that changes in the death of ovarian follicular cells and immune cells have the same orientation and similar in quantitative terms. This allows us to judge the ovarian cell death in patients according to apoptosis and necrosis of immune cells from peripheral blood. Analysis of our data regarding the death of cumulus and immune cells indicates an important indicator to find effective means for the treatment and prevention of immune-mediated diseases, including autoimmune disorders ovaries: these features have influence on necrotic cell death, reducing both primary and secondary, postapoptotychnyy, necrosis. Identified changes in the functional state of mitochondria, levels of proinflammatory cytokines and apoptotic cell death of the reproductive and immune systems under experimental immune ovarian damage may be the main mechanisms that regulate the functional state of the reproductive system.