Karpenko O. Scientific principles of the development of biotechnologies of surfactants with polyfunctional properties

The thesis is devoted to the technology and application of surface-active products of microbial synthesis of the genera of bacteria Gordonia, Rhodococcus, Pseudomonas, Bacillus (biosurfactants). The technologies of microbial biosynthesis of complex surfactants of different nature and composition of various localization: extracellular complexes rhamnolipids and polysaccharides of bacterium Pseudomonas, cell-bound trehalose lipids with fatty acids and carotenoids, as well as extracellular polysaccharide emulsifier of actinobacterium Gordonia, were scientifically substantiated. The product yields were significantly decreased via the optimization of the synthesis conditions for biosurfactant complexes by mathematical modeling and scaling processes on fermentation equipment. A rational methods for the isolation of biosurfactant complexes were proposed, a number of effective forms of the products were produced, their physico-chemical and biological properties (emulsification, solubilization, foaming activity, effects on the permeability of cell membranes, antimicrobial, antiviral effects) were determined. The technologies of the application of biosurfactant complexes in the field of the production of enzymes, pharmaceuticals, detergents, metal corrosion inhibitors, plant growth regulators, to enhancers of the activity of fertilizers and biocides, environmental remediation, enhanced oil recovery and energy saving technology of vegetable oil refining.