Kmet T. Pathogenesis of selective sensibility of various portions of the neocortex to ischemic-reperfusion injury of rats with streptozotocin-induced diabetes mellitus

Object -is ischemic and reperfusion injury of the cortex of the frontal, parietal and temporal lobes of the cerebral hemispheres on the background of streptozotocin-induced diabetes mellitus; goal - is to establish the mechanisms of selective sensitivity of neurons and glial cells of the cortex of the frontal, parietal and temporal lobes of the cerebral hemispheres, as well as the endotheliocytes of their vessels to ischemic-reperfusion injury in rats with experimental diabetes mellitus; methods - pathophysiological; immunocytofluorescence; densitometric; computer-mathematical classification analysis of images; morphometric; statistical-analytical; results - the mechanisms of selective sensitivity in the cerebral cortex of the frontal, parietal and temporal lobes to incomplete global ischemia-reperfusion of the brain in the dynamics of its course in male rats with streptozotocin-induced diabetes mellitus have been studied. The assessment criteria of the cellular sensitivity in the above mentioned cerebral cortex lobes to ischemia-reperfusion were: density of location per square unit of the endotheliocytes of the vessels, nerve and glia cells without the signs of injury and with apoptosis signs, the density of location of р53+-, Bcl-2+-, Hif-1α+-neuro- and glia cells, protein content in them expressing these cells, and cellular DNA; the results of the study have demonstrated availability of both common and different pathogenic links of injury in the nerve, glia and endothelial cells in the cerebral cortex of the frontal, parietal and temporal lobes of the hemispheres caused by diabetes mellitus, ischemia-reperfusion of the brain and association of these pathological conditions. A modifying effect of diabetes mellitus has been demonstrated on the indices of cortical sensitivity of the examined lobes to ischemia-reperfusion of the brain both in the early and late post-ischemic periods; on the basis of the data obtained the gradation of the nerve and glia cells in the cortex of the examined cerebral lobes to ischemia-reperfusion of the brain has been made in the dynamics of the latter in animals without diabetes and with this pathology, differences of leading mechanisms in realization of cellular sensitivity to ischemia-reperfusion in case of absence or availability of diabetes mellitus; according to the examined indices in rats without diabetes mellitus the basis of sensitivity realization to ischemia-reperfusion is formed by disorders of interrelations in the system р53-dependent processes of apoptosis/Bcl-2-dependent anti-apoptotic processes, deeper in case of diabetes, and instability of induced hypoxia of Hif-1α transcription factor in animals with diabetes.