Antonyan I. Application of bone marrow stromal cells for the treatment of erectile dysfunction in androgen deficiency (experimental and clinical reserch)

The dissertation is devoted to the study of the effectiveness and safety of use of bone marrow stromal cells (BMSS) for the treatment of erectile dysfunction in androgen deficiency. In the experimental part of the study with the use of sexually mature male rats, a model of experimental androgen deficiency was developed by intraperitoneal administration of a gonadotoxic chemical compound - cadmium chloride (CdCl 2) in a dose of 150 ?g / 100 g of animal body weight. The developed model was characterized by the development of the necessary gonadotoxic effect with a steady decrease in the concentration of total testosterone in the blood and inhibition of the copulative behavior of animals in the absence of a significant overall toxic effect. On the model of experimental gipogonadism, the results of its treatment with BMSS have been studied and the effectiveness of their intratesticular administration in both testicles at 200,000 cells has been proved. In the separated period during the histological examination of genital organs, signs of oncological transformation were not detected. In the clinical part of the work for the first time, clinical testing was performed on the method of intratesticular transplantation of autologous BMSS for the treatment of erectile dysfunction in patients with androgen deficiency on the background of type 2 diabetes with overweight. It has been proved that cellular therapy with the use of autologous BMSS in combination with treatment of diabetes and measures aimed at normalization of weight contributes to the restoration of indicators of erectile function, the concentration of total testosterone in the blood, along with the normalization of carbohydrate and fat metabolism. Side effects during the study are not recorded.