Shulga S. The creation of transport nanosystems based on essential phospholipids of sunflower oil to enhance the biological effects of curcumin

The manuscript presents a comprehensive study on the development of technology of dry deoil lecithin (mixtures of phospholipids) from phosphatide concentrate of sunflower oil, with subsequent use as a raw material for creating liposomal forms of pharmaceutically active ingredients. It was determined that liposomes from sunflower phospholipids are more stable to initiated oxidation than liposomes from soybean and egg yolk phospholipids. It is shown that the stability of liposomes from sunflower phospholipids is due to the presence of chlorogenic acid (0.8%) and α-tocopherol (0.07%). The most effective method of creating a liposomal form of curcumin is determined - the method of extrusion through membranes with the efficiency of curcumin incorporation 95-98%. A new approach and treatment strategy for Alzheimer's disease is proposed, taking into account the key role of the liposomal form of curcumin. It has been established that curcumin can interfere with the formation of Aβ-monomers by interacting with the processing sites of the precursor of β-amyloid peptides. A number of curcumin derivatives, in particular 9-keto 11-enol curcumin I, have been found to have a higher binding energy (affinity) to Aβ than others. The possibility of using 9-keto 11-enol curcumin I as an inhibitor of Aβ aggregation (therapeutic effect), which is considered to be the first step in the development of Alzheimer's disease, has been scientifically substantiated. Liposomal curcumin nasal therapy with a preclinical model of Alzheimer's disease (intrahypocampal administration of Aβ42 homoaggregates) has been shown to inhibit angiotensin converting activity and show greater anticytokine potential in the hippocampus in animals. The addition of curcumin removed the initiating effect of Aβ42 on the mononuclear pool of proinflammatory cytokines (IL-1β and TNFα) and restored the intracellular pool of anti-inflammatory interleukin IL-10.