Oblap R. Methodology of molecular genetic evaluation of food safety and quality

Research is dedicated to development of methodology of molecular-genetic evaluation of quality and safety of agricultural products of plant and animal origin. Complex research of polymorphism of Quantitative Trait Loci was performed. DNA sequences of animal, plant and microorganisms` genome regions were analyzed. PCR-based diagnostic kits were developed, implemented and offered for use. Genetic structure of two Black-and White Ukrainian Dairy cattle populations was investigated. Polymorphism of genes κ-casein, β-lactoglobuline and prolactin associated with high productive traits were studied in these two populations (n=451). The effect of different genetic variants of CSNΚ, BLG and PRL combination on cheesemaking properties of milk was studied as well. For the improvement of identification of dangerous infection agents in food of animal origin the comparative diagnostic on BLV was done in cattle by means of Real-Time PCR and RID methods. Conservative region of Env gene which encodes viral glycoprotein gp51 was used as molecular genetic marker. Efficiency of using milk as alternative bio-material for BLV diagnostics was evaluated. New diagnostic kit was proposed as additive tool for antileukemic measures in farms and for milk testing at milk processing enterprises of Ukraine. Using molecular genetic markers of 16S rRNA regions and Tu elongation factor genes to identify bacteria of the genera Bifidobacteria, Lactobacillus and Enterococcus a method based on SYBR®Green technology was developed to determine beneficial microflora in dairy products. Possibility of lactic acid bacteria and bifidobacteria identification in dairy products during the control of the microflora content as well as during the creation of new yeasts, probiotics and probiotic products was proved.