Balitska O. Pathogenetic role of oxidative and immuno-cytokine disorders in the development of periodontitis in diabetes

The thesis provides theoretical generalization and new solution to the current scientific problem, which is to clarify the role of oxidative, cellular and humoral links of adaptive immunity and immunocytokine disorders in the mechanisms of development and course of generalized periodontitis in patients with type 2 diabetes. Based on a comprehensive study of pathogenetic patterns of development and course of generalized periodontitis in patients with type 2 diabetes proved the initiating and leading role of free radical oxidation and disruption of the body's antioxidant defense system in their mutually burdensome course. In this case, chronic periodontitis on the background of type 2 diabetes mellitus is accompanied by tension of antioxidant reserves with the maximum increase in protein and lipid peroxidation in both serum and oral fluid, which is confirmed by the probable relationship between periodontal indicators and indicators of periodontitis. It was supplemented data on the features of cellular and humoral adaptive immunity in patients with generalized periodontitis on the background of type 2 diabetes mellitus, which are characterized by an imbalance of subpopulations of T and B lymphocytes with a decrease in the main populations of lymphocytes with phenotype - CD3 +, CD4 +, CD8 + and increased with confirmed by a decrease in the immunoregulatory index (CD4 + / CD8 +) in the blood of patients relative to control (p<0,05). Updated and supplemented the probable interactions between periodontal changes and local humoral immunity (negative links between PMA-sIgA and IR-sIgA, as well as direct links between PMA-IgG and IR-IgG) in generalized periodontitis on the background of diabetes mellitus 2 type under the condition of significant changes in the humoral part of the adaptive immune system in both blood and oral fluid. It was confirmed and supplemented that the development of pathological changes in the oral fluid as a factor of natural immunity in patients with generalized periodontitis combined with type 2 diabetes depends mainly on the intensity of generalized periodontitis. Positive correlations of average strength in patients of the comparative group with GP were clarified and supplemented: between the content of IL1β and sIgA (r = 0.46), Ig G (r = 0.32), TBA-AP (r = 0.36) , p<0.05 and between the level of IL10 and sIgA (r = 0.48), Ig G (r = 0.34), TBA-AP (r = 0.39), p<0.05, in oral fluid. A strong positive correlation was found between the level of IL1β and sIgA (r = 0.89) and IL10 and sIgA (r = 0.82), p, p1<0.01, in the oral fluid of the examined main group (type 2 diabetes + GP). In this group of patients were determined positive correlations of medium strength between the values of IL1β and Ig G (r = 0.43), p<0.01, p1<0.05, TBA-AP (r = 0.37), p<0.05, p1> 0.05 and IL10 and Ig G data (r = 0.45), p, p1<0.05, TBA-AP (r = 0.40), p<0.05, p1 > 0.05.