Petryk N. Pathogenetic substantiation of the possibility of using MSCs for the prevention of chronic inflammation

The dissertation presents a theoretical generalization and solution of an actual scientific medical problem – pathogenetic substantiation of the use of mesenchymal stem cells for the prevention of chronic inflammation. The model of secondary chronic inflammation shows that according to the main parameter of inflammation - the reactions of the blood system, the use of mesenchymal stem cells significantly reduces the chronicity of inflammation. In the reproduction of chronic inflammation on the background of the use of mesenchymal stem cells, in contrast to the natural course, the number of leukocytes is lower during almost the entire experiment. The maximum number of lymphocytes in the natural course of chronic inflammation was observed on the 28th day (significantly higher by 2.6 times p <0.01), and in inflammation, on the background of the introduction of mesenchymal stem cells there is a shift in the 5th and 10th day ( respectively, 1.9 times p <0.05 and 2.8 times p <0.01), which contributes to the earlier end of the inflammatory reaction. For the first time, a significant anti-inflammatory and immunomodulatory effect of mesenchymal stem cells under conditions of secondary chronic carrageenan inflammation were established. It is manifested by various components of the cell-tissue reaction of the inflammatory focus: leukocyte infiltration, a proliferation of connective tissue elements. For the first time, it was found that the introduction of allogeneic bone marrow-derived mesenchymal stem cells showed a significant decrease in IL-6, TNF-α, and CRB in the plasma of animals of the control group with mesenchymal stem cells without causing inflammation compared to the control group. This can be explained by the immunomodulatory ability of mesenchymal stem cells. The practical significance lies in the fact that the patterns of changes in the reactions of the blood system in the dynamics of secondary chronic inflammation, which occurs against the background of the introduction of mesenchymal stem cells.