The dissertation presents research materials on the analysis of the epizootic situation of infectious agalactia of sheep and goats in farms in the south of Ukraine. In nine southern districts of Odesa region, the incidence of infectious agalactia of sheep and goats in 2016 was 13.1%, in 2017 - 11.1%, in 2018 - 10.1%.
The highest incidence of infectious agalactia of sheep and goats in 2016 was in Artsyz - 23.5% and Tarutyn districts - 21.7%. In the Bolgrad district the incidence rate was 16.5%, in the Reni district - 10.2%. The lowest incidence rate was registered in Izmail district - 5.9% and Tatarbunary district - 1.2%.
In nine southern districts of Odesa region, the incidence of infectious agalactia of sheep and goats in 2016 was 13.1%, in 2017 - 11.1%, in 2018 - 10.1%.
The highest incidence of infectious agalactia of sheep and goats in 2016 was in Artsyz district - 23.5% and Tarutyn district - 21.7%. in Bolgrad district incidence rate was 16.5%, in Reni district - 10.2%.
In 2017, the highest incidence rate was registered in Artsyz district - 20.8%, in Tarutyn district - 18.6%, in Bolgrad district - 12.7%, in Tatarbunary district - 10.9%, in Reni district - 7.9%, in Saratsky district - 6.2%, and in Izmail district- 4.4% and B.‒Dniester district - 0.6%.
In 2018, no clinical signs of infectious agalactia of sheep and goats were registered in Kiliysky, B.‒Dniester and Tatarbunary districts of Odesa region. Instead, in Tarutyn district the incidence rate decreased by 6.9% and amounted to 14.8%, compared to 2016, in Saratsky district - by 4.4% (4,5 %), in Bolgrad district - by 3.6% (12,9 %), in Artsyz district - by 3.2% (20,3%), in Reni district - by 2.6% (7,6 %) and in Izmail district - by 2.1% (3,8 %).
"Vaccine inactivated against infectious agalactia of sheep and goats" was made by inactivating the accumulated bacterial mass with 1% formaldehyde. The obtained raw material was precipitated by centrifugation and washed twice with sterile phosphate-buffered saline. A suspension with a concentration of 1x108 CFU/cm3 was made from the precipitate. To the prepared bacterial mass was added aluminum hydroxide in the ratio: 60% suspension of inactivated cells of the production strain Mycoplasma agalactiae S-11 (6x107 CFU in one dose) in sterile phosphate-buffer solution and 40% aluminum hydroxide.
It was found that the strain of Mycoplasma agalactiae S-11, which is part of the vaccine, has pronounced antigenic properties, and the vaccine made from it is able to activate in the body of vaccinated animals the production of specific antibodies in high titers. Double immunization promotes the development of protective antibody titers from 3.54 to 3.59 log2, which ensures the immunity of animals to the disease in conditions of joint keeping of vaccinated and sick animals.
It was found that vaccination of sheep with inactivated vaccine against infectious agalactia of sheep and goats increases morphological parameters of blood: hemoglobin content increased by 6.3% (106,1±3,2 g/l, p˂0.05), compared to control (99.8±3.6 g/l), the number of leukocytes - by 23% (10,7±0,4 G/l, p˂0.001), the number of lymphocytes - by 5.8% (49,2±2,2 %, p˂0.05). A positive correlation between the content of leukocytes and the absolute number of lymphocytes indicates the activation of the immune cell.
