Pobielienskyi K. Cryodestruction of thyroid gland with diffuse hyperplasia on the background of arterial hypertension (experimental study)

The object of the research was destructive and restorative processes in thyroid tissue after administration of propylthiouracil and cryoablation in different regimens in rats with arterial hypertension. The research was goaled to study the influence of hypertension on the cryoablation outcome in normal and pathologically altered thyroid tissue of rats, as well as optimization of the regimen of the diffuse hyperplastic thyroid gland cryoablation and evaluation of the rate of reparative processes in the thyroid residue after cryoablation. Research methods were as follows: modeling of diffuse hyperplasia of the thyroid gland, its cryoablation and resection, contact thermometry, ELISA, histological, morphometric, immune histochemical, biochemical analysis, methods of statistical analysis. For the first time, it was found in the experimental model that the state of hypertension did not affect the result of cryoablation of the thyroid gland in intact animals. However, after the manifestation of pathological changes characteristic of propylthiouracil-induced hyperplastic processes, there was an increase in the resistance of the thyroid tissue of hypertensive animals to cryoablation. For the first time, the influence of differences in thermophysical characteristics of pathologically altered by propylthiouracil thyroid tissue on parameters of its cryoablation was determined, as well as the profile of histological changes of thyroid gland with diffuse hyperplasia observed after different regimens of the cryoablation. It was determined that hyperplastic thyroid tissue of hypertensive rats was more resistant to cryoablation in the used regimes in comparison with normotensive rats. It has been proven for the first time that the processes of neofoliculogenesis in thyroid tissue were less active after cryoablation than after resection. For the first time, it has been established that the efficiency of cryoablation can be increased by using contact heating of the tissue in the "freeze-heating" cycle. Field of Application: cryomedicine, medicine, endocrinology, biotechnology, veterinary.