Skrynchuk O. Pharmacognostic study of Crambe cordifolia Stev. and Crambe koktebelica (Junge N. Busch)

The thesis presents the results of a complex comparative pharmacognostic study of leaves and roots cultivated in Ukraine, two species of the genus Crambe L. of the cabbage family Brassicaceae – Crambe cordifolia Stev. and Crambe koktebelica (Junge) N. Busch). Morphological and anatomical analysis of the leaves and roots of Crambe cordifolia and Crambe koktebelica was carried out, the main diagnostic macro- and microscopic signs were determined. Projects of quality control methods (QCM) “Leaves of Crambe cordifolia”, “Roots of Crambe cordifolia” were developed. The optimal conditions for obtaining thick extracts from the leaves and roots of Crambe cordifolia and Crambe koktebelica were determined, for which the QCM projects “Thick extract of the leaves of Crambe cordifolia”, “Thick extract of the roots of Crambe cordifolia” were developed. Acute toxicity of thick extracts of the leaves of Crambe cordifolia and Crambe koktebelica was established, which according to K. K. Sydorov classification are referred to the toxicity class VI – almost harmless substances, LD505000 mg/kg. A pharmacological study of thick extracts of the leaves and roots of Crambe cordifolia and Crambe koktebelica was performed, the presence of hepatoprotective and antioxidant activity was established. The studied thick extracts at a dose of 100 mg/kg showed a hepatoprotective effect, which was somewhat inferior to the activity of the Silibinin comparison drug. It was studied the antioxidant activity of thick extracts from the leaves and roots of Crambe cordifolia and Crambe koktebelica in vitro. Thick leaf extracts of both species of Crambe compared to thick root extracts showed a better ability to inhibit the DPPH radical. Their IC50 values were (358.27 ± 2.78) μg/mL and (374.18 ± 3.57) μg/mL, respectively.