Radchuk V. Genetic tranformation in Brassicaceae with genes of nisin biosynthetic pathway and their effective combination in transgenic plants.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U003171

Applicant for

Specialization

  • 03.00.15 - Генетика

30-11-2000

Specialized Academic Board

Д 26.202.01

Essay

Dissertation is devoted to the establishment of plant genetiс transformation for cultivars of oil-seed rape, cabbage and cauliflower with the genes of biosynthetic pathway of nisin and the study of transferred genes in the transformed plants. Used methods are transformation by using of Agrobacterium, direct gene transfer into mesophyll protoplasts, molecular biological methods for analysis of transgenic plants. It is established that effective combination of transgenes is possible after their stable integration into plant genomes. Multiple tranfer of genes is demonstrated after transformation of Arabidopsis with several construction simultaneously. This transfer did not depend on the length of transgenes.

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