Luzhetskyy A. Gene cloning system for actynomycetes strains - producers of kanamycin Streptomyces kanamyceticus 1 and landomycin E Streptomyces globisporus 1912.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0403U003395

Thesis Registration Form

0403U003395.pdf

Applicant for

Specialization

  • 03.00.15 - Генетика

16-10-2003

Specialized Academic Board

Д 26.212.01

Insninute of plant physiology and genetics

Essay

The thesis is devoted to the development of effective method of introducing of recombinant plasmids in producers of kanamycin Streptomyces kanamyceticus and of landomycin E Streptomyces globisporus 1912 using intergeneric conjugation from E.coli. Gene cloning system was developed for 16 species of actynomycetes, which are low frequency transformed with plasmid DNA. The efficiency of the method was shown for obtaining of recombinant starins by means of directed mutagenesis and heterologous expression.

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