Melikhov S. Brucella ovis changeability, genus-specific and species-specific antigenicity, and ovine epididymitis (Brucella ovis) and ovine chlamydiosis serological diagnostics at a mixed current

Object - Brucella ovis-infection and Chlamydiosis of ewes, Brucella strains (Brucella abortus, Brucella melitensis, Brucella suis, Brucella ovis), breeding flocks of rams unsuccessful on ovine epididymitis and chlamydiosis, B.ovis and Chl.psittaci antigens and diagnostics Serum's. The purpose - definition of variability of the Brucella ovis strains, genus-specific and species-specific antigenicity of the agent of an infectious epididymitis of rams and features of serological diagnostics Brucella ovis-infection and chlamydiosis at a mixed current. Methods - epizootological, clinical, serological, bacteriological, allergic, statistical. Instrumentation - microscope, thermostat, pH-meter, centrifuge, self-acting spectrophotometer. Theoretical and practical results and novelty - was spotted variability of morphological and antigenic properties of the strains B.ovis, which duration time growing on dense mediums, are improved differential tags at differentiation of Brucella cultures, the Brucella ovis strains with high genus-specific and species-specific of other Brucellas viewers 2 groups are detected which is conditionally designated as R-a (R-abortus) and R-m (R-melitensis). Are theoretically justified and the expedients of obtaining patrimonial brucellous and specific R-Serum's with application selected of the strains B.ovis designed. Fixed and the nature of non-specific modulation of the immune answer on antigens of Chlamydia is described at introduction of animal brucellous antigenes, in particular, Brucellin VIEV. Are offered the industrial strains B.ovis with the different antigenic performance for manufacture of diagnostic kits. The dependence of a level positive reaction and level of procreation is detected. A degree of implantation - normative documentation on manufacture, check and application " Kit of builders for serological differentiating of Brucella cultures ". The kit will be utilized in practice of state laboratories of veterinary medicine.