Rublenko N. Detection of Virulence Factors of Salmonella spp. by a Polymerase Chain Reaction

The subject of this study is a prevalence of Salmonella spp. in Ukraine during 2006–2019. The prevalence of Salmonella serovars in different regions is analyzed, namely, the agents of fowl typhoid and Pullorum disease, as well as non-typhoid salmonellosis. In polymerase chain reaction we detected the genes encoding virulence factors: invasion (invA), adhesion (agfB, sefA), endotoxins (prt), colonization (sopE, gipA, sodC1). Also, genes conferring resistance to tetracyclines (tetG), sulfonamides (sulI) and class 1 integron In104 – a mobile genetic element that localizes on the chromosome, have been detected. The In104 integron contains functional elements that replicate antibiotic resistance genes. We determined susceptibility to antimicrobials of class β-lactams, aminoglycosides, dihydrofolate reductase inhibitors, phenicols, and quinolones. Induction of temperate bacteriophages was performed using the chemotherapeutic drug Mitomycin C. A set of primers was constructed to identify bacteria of the genus Salmonella and serovars Enteritidis and Typhimurium.