Postoi V. Obtaining Transfer Factor from Lymphocytes of Bovine Colostrum and its Adaptogenic Properties

The dissertation is devoted to the improvement of the method for obtaining transfer factor from colostral lymphocytes and the studying of its adaptogenic properties. In the dissertation, in accordance with the set goal, it is experimentally substantiated the obtaining of biologically active samples of transfer factor specific for Salmonella enterica serovar Dublin; its immunomodulatory and protective effect regarding diseases of the digestive tract in newborn calves under industrial conditions has been confirmed. For the first time, a transfer factor specific for the causative agent of salmonellosis (Salmonella enterica serovar Dublin), which induces an active cellular immunity in allogeneic and xenogeneic recipients, was obtained from colostral lymphocytes of sensitized cows. A rational scheme for sensitization of donor cows for transfer factor specific for the causative agent of salmonellosis has been developed. Using an improved technique, a transfer factor specific for the causative agent of salmonellosis in calves, which is able in vitro and in vivo to transfer the state of sensitization to recipients and which does not have a species specificity. The transfer of delayed-type hypersensitivity reaction to the causative agent of salmonellosis in calves from sensitized cows to intact white mice was performed. The positive effect of the obtained samples of transfer factor from colostral lymphocytes on postnatal adaptation in calves is proved. The application of transfer factor contributes to the shift of the balance between the cellular and humoral parts of the immune defense towards the activation of its cellular immunity, which indicates the stimulating effect of the transfer factor on the immune response of the cell type. For the first time, the stimulating effect of transfer factor on phagocytic activity and phagocytic index of blood lymphocytes in calves was established. The application of transfer factor in combination with anti-dehydration and antimicrobial means helps to increase the resistance in calves. The results of the dissertation research might be implemented as elements of biotechnology during industrial production of drugs based on transfer factor specific for salmonellosis and other infectious diseases that is derived from colostral lymphocytes.