Sukhomlinova I. Role of the spleen in the regulation of erythopoiesis due the influence of serotonin on the processes of free-radical oxidation.

An object is the level of products of free-radical oxidation, activity of the enzymes of the antioxidant system and serotonin in the cortical and cerebral matter of the rats' liver in the conditions of stimulation and inhibition of erythropoiesis. The aim is to set the role of the oxidant-antiooxidant system of the liver tissue, the spleen and endogenous serotonin in the mechanisms of regulating the erythropoiesis. The methods are laboratory, biochemical, cytomorphological, statistical. Results: a new method of quantitative determination of serotonin and melatonin in one test of biological material is developed; the obtained results enable to estimate newly the cooperation of the system of blood formation and the system of blood circulation, on the basis of what the origin of some forms of the anaemic states at hyperserotoninemia and splenectomy becomes clear. Novelty: for the first time both the kinetics of erythropoiesis in the conditions of its stimulation and inhibition and the state of oxidant homeostasis at these processes in the kidney tissues of rats are studied in complex; the findings about the direct influence of oxidant homeostasis of kidney tissues on the mechanisms of regulating the erythropoiesis are obtained; for the first in the experiments in vivo and in vitro it is shown, that serotonin in the concentrations near to physiological is an antioxidant and, thus, takes part in regulation the erythropoesis; it is established that the concentration of serotonin of blood and kidneys depends on blood-filling of the spleen: at depositing in it the excess of blood increases, and after splenectomy - it decreases; the spleen which has lost the opportunity to deposit the excessive blood is unable to increase the level of serotonin in blood at post-transfusion erythropoesis; it is shown that through the depositing function of the spleen the cooperation between the systems of blood formation and blood circulation is provided. It is introdced in the educational process of the Departments of Normal Physiology of the Kharkov State Medical University, Ukrainian Stomatology Academy, Dnepropetrovsk State Medical Academy. It is used in the field of medicine.