Tryapitcyna N. DNA-fragments polymorphism and genome organization peculiarities of some mammals and dicotyledonous plants

The thesis covers the problems of ISSR-PCR- and IRAP-PCR markers utilization for genetic diversity quantification of plants and animals domesticated and closely related wild species. Correlation between ISSR-PCR-markers polymorphism level and thermodynamic characteristics of its microsatellite flank loci was found. The most suitable ISSR-PCR-markers for cattle intrapopulational genotyping as well as for phylogenetic studies of subfamily Bovinae representatives were selected. Fact, that the ISSR-PCR-markers flanked by the microsatellite loci with high thermodynamic characteristics are more suitable for wild soybean genotyping and with low one - for soybean cultivars quantification were shown. he non-neutrality of the ISSR-PCR- and IRAP-PCR-markers, which were considered to be neutral for the selection, was demonstrated for the cattle.