Belokon' V. Development of Laboratory Regulation of Cultivation of the Vaccine Strain LK-М as Infection Agent of Classical Swine Fever

The thesis is devoted to the development of the culture method of the vaccine strain LK-М as infection agent of classical swine fever.Due to the thesis investigations the main techniques of the primary obtained cell culture from lambs' testiculus were optimized and the hystochemical kind of ELISA for the detection and titration of the CSF virus in cell culture was created. The working seed of the cell culture of lambs' testiculus was obtained and its main biological properties and sensibility to virus were described. There were scientifically grounded the methodology of the virus material obtaining (strain LK-M of CSF virus), being sufficiently concentrated. The immunogenecity of the strain LK-M of CSF virus was researched, being obtained from heterologic cell culture of lambs' testiculus. There were described its influence to some cell immunity parameters in swine.