Wajdi K. Effect of erythrocyte membrane and shape modificators on some functional properties of the cells

The object of study is erythrocetes membran of I and II groop blood. The aim of study is to clarify the role of cell shape as a factor determining susceptibility of cells toward damage action of lytic peptides melittin and its analog [Ala-14]melittin (P14A), resistance toward acid hemolysis, the transforming action of various chemical reagents, and the effect of such modification on gallium-induced aggregation. Methods jf stady: spectrophotometry, spectrofluorometry, differential centrifugation. Theoretical and practical resultes, innovation: The action action of chemicals on kinetic of shape transformation of erythrocytes was investigated and the sequence of shape transformation potential of substances was obtained in the next form: spermidine <guanidine <p-CMBS <NEM <CCCP <A23187 <DNDS <DIDS <SITS <lysolecithin <Cpr <SDS <CTAB. It has been shown that an ability of modifiers to change acid resistance of cells is attributed by their ability to affect ion permeability of membrane. The substances that able to reduce membrane permeability demonstrate inhibitory effect on acid hemolysis whereas those increasing permeability show activating one. The substances having no effect on permeability irrespective to their influence on cell shape had no effect on acid resistance of cells. Studying the effect of modifiers showed that A23187, SDS and DIDS had a maximal effect on aggregation of erythrocytes induced by gallium. These substances changed substantially dispercity of aggregates i.e. their size distribution reducing the size of aggregates. Field of application: in cell phisiology, medicine, pharmacology scientifical research, in educational process of phisiology and biological chemistry