Fedoryshyn M. Genetic control of Landomucin А saccharide part developing

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0407U001229

Thesis Registration Form

0407U001229.pdf

Applicant for

Specialization

  • 03.00.15 - Генетика

20-03-2007

Specialized Academic Board

Д 26.202.01

Essay

We consequently have inactivated glycosyltransferase genes (lanGT2, lanGT1, lanGT4, lanGT3) to show hexasaccharide chain developing step by step. The olivosyltransferase LanGT1 catalyzes the transfer of the second and the fourth sugar, the rhodinosyltransferase LanGT4 attaches the third and the sixth sugar during the biosynthesis of the landomycin A hexasacharide side chain. This is the first description of glycosyltransferases (LanGT1 and LanGT4) involved in natural-product biosynthesis and are working iteratively on a growing saccharide chain. Putative NDP-hexose synthase LanZ2 is required for the smooth and complete biosynthesis of the hexasaccharide part of landomycin A

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