Grushka N. Involvement of thromboxane in the cell death of cultured rat hepatocytes

Тhromboxane (TX) is known to have injurious effect on a liver, but its direct influence on pathways of cellular destruction (apoptosis, necrosis or autophagic death) is not investigated enough. It was established, that exogenous ТхB2 increased the amount of hepatocytes with morphological features of early stages of apoptosis (chromatin condensation), caused activation of caspase 3. Furthermore, TxB2 did not affect necrosis and the intensity of autophagy. Treatment of cultured hepatocytes with Тх and cyclosporine A, decreased the amount of apoptotic cells. This indicates on participation of mitochondria in modulation of apoptosis by Тх. Tx B2 intencified the action of carbon tetrachloride on hepatocyte apoptotic death and increased chromatin condensation. Тх В2 treatment of hepatocytes injured by chenodeoxycholic acid, increased the amount of cells with a final stage of apoptosis. Inhibitor acetylsalicylic acid and benzylimidazole decreased hepatocytes apoptosis, induced by ССl4, and did not change apoptosis, induced by CDCA.