Shutchenko P. Immunohistochemical diagnostics and estimation of cell immunity at salmonellosis of hens.

Object of research: hen salmonellosis and immunohistochemical methods diagnostics of this disease. The aim of research: to determine quantity changes of main clusters of spleen and bursa of Fabricius immunocompetent cells in dynamics of immunity formation at chickens after infection with salmonella or vaccination with attenuated vaccine against this disease using modern immunohistochemical methods, to develop an immunohistochemical technique of salmonella antigenes revealing, places of their localization in organs of chickens infected with a salmonellosis. Methods of research: serological, bacteriological, biochemical, pathohistological, immunohistochemical and statistical. The equipment: thermostate, cryotome, microtome, freezer. Theoretical results: The opportunities of immunohistochemistry as sensitive and exact estimation method of cell and humoral immunity state are determined. The expediency of immunohistochemical method using for diagnostics of hen salmonellosis. Practical results: It was developed and tried the diagnostical method of hen salmonellosis by immunohistochemical research of avian slaughter products on salmonella contamination and the recommendations for definition of immunity state under the cluster content at salmonellosis was introduced. "Methodical recommendations for immunohistochemical diagnostics and immunity estimation at avian salmonellosis" are developed. The novelty of work is confirmed by 2 patents. Degree of inculcation: "Methodical recommendations for immunohistochemical diagnostics and immunity estimation at avian salmonellosis". Field of using: veterinary medicine.