Degtyar'ov I. Study proteinogenic characteristics of M. bovis and improvement the metod to produce purified tuberculin (PPD) for mammal.

The Object of the studies: tuberculosis, tuberculin. The Methods of the studies: эпизоотический, allergic, biochemical, clinical, bacteriological and statistical. Novelty - is chosen and selection proteinogenic strain M bovis, is received patent of the Ukraine 25807 on useful model "Tuberkulinogenny strain M bovis 4" and certificate State scientifically-checking institute to biotechnologies and strain microorganism 425. The Advanced way of the sowing and adaptation proteinogenic cultures on syntetic nourishing ambience. It Is Installed that tuberculin, received by method filtration with use membrane fractionating molecules protein tuberculin's with molecular mass from 10 before 300 kDa on biological activity does not yield tuberculin (PPD) for mammals in standard solution, is advanced technological scheme of the fabrication tuberculin's cleaned (PPD) for mammals in condition biofactory. Is it for the first time received and studied biological characteristic tuberculin from disintegrated bacterial massproteinogenic cultures M bovis IEKVM-1. I is proved that this tuberculin does not possess the sensitization characteristic, specific and possesses the high biological activity. The Designed methods of the determination of the fullness inactivation and biological activity tuberculin (PPD) for mammals according to international requirements. The Material to thesis's are used in scholastic process in program of the lecture course and laboratory practical occupation Harkov state zoo veterinary to academies. The Profession - veterinary medicine.