Skoryk M. Functional Conditions of Erythrocytes and Trace Element Contents in the Internal Organs of Laying Hens under the Influence of Substances of Humic Nature

The subject of research: the functional condition of erythrocytes and the trace element contents in the inner organs of laying hens in the second stage of production. The purpose of research: to perform investigations into the functional condition of erythrocytes and the contents of cations (Fe, Cu, Mn, Zn) in the organisms of laying hens in the second stage of production and the effects produced by biologically active feed additives of huminous origin “Hydrohumat” and “Humisol-T-2”. Methods and equipment: clinical, physiological, hematological, biochemical, zoo-engineering, mathematical; Goryayev’s apparatus, microscope, photoelectric colorimeter of KFK-3-01 Model, nuclear-absorption spectrophotometer of AAS-3 Model. Theoretical and practical results, scientific novelty: It has been revealed that the functional condition of erythrocytes in laying hens during the phase of physiologically retarded egg-forming processes considerably improves due to the fact that the functioning of the antioxidant protection system in birds is enhanced by the action of biologically active feed additives of huminous origin. It has been shown that in their second productive phase the laying hens, due to the effects produced by “Hydrohumat” and “Humisol-T-2”, display increased contents of copper and zinc in liver tissues and more iron and zinc in the tissues of ovaries, but the contents of iron and zinc in the tissues of spleen are decreased; it has been also established that there is a correlation between these indices and the morpho-functional condition of erythrocytes. The outcome and the scope of implementation: Recommendations were worked out on the use of feed additives of huminous origin in the rations for laying hens in their second productive phase with an aim to improve the physiological status of poultry organisms and to prolong egg-forming processes (Dnipropetrovsk Regional Administration of Veterinary Medicine, Protocol No. 4 dated October 23, 2007). Proposals: It was suggested that the biologically active feed additives be used as supplements to the rations for laying hens in their second productive phase in the dosage of 1.8 ml of “Hydrohumat” and 60 ml of “Humisol-T-2” per 1 kg of feed mixture twice a day during 21 days at 21days’ interval. Practical implementation: The suggested method has been adopted on the poultry farms of “Argocentr” PLC. in Krasnopoliye and Dniprodzerzhinsk and in “Ptitseprom” Agricultural Enterprise in the town of Tomakovka, Dnipropetrovsk Region. The fields (branches) of application: poultry farming, veterinary medicine, physiology of animals.