Zabelina V. Parthenocloning of the Silkworm by Donor Ovary Cultivation in the Male Host

Research object: Determination of the thermoparthenogenesis ability of the eggs developing in implanted ovary. Inheritance and variability of parthenogenetic progeny developed from implanted ovary. Purpose: detection and phenotypic evaluation of the parthenocloning ability of silkworm eggs, that developed in the ovaries implanted into visceral cavity of opposite sex larvae. Research methods: genetic analysis, microsurgical method of gonad transplantation, thermoparthenogenesis method by Astaurov, cytogenetic method for research of oogenesis and spermatogenesis, electrophoretic protein separation in linear gradient of polyacrylamide, statistic data analysis. Theoretical and practical results, novelty: First we transplanted ovaries into the male hosts in different larval stages to analyze the ontogenetic forming of parthenocloning ability of female germ cells It was discovered that genetically determined donor capacity for parthenocloning hasn't decreased considerably by cultivating in male recipient even during several larval stages. It was detected that the frank phenotypical variability (dropout of diapause, semicomplete colouration character by larvae in I stage and cocoons) may occur in a clone under its cultivation in a male host. The place of oocytes' ontogenetic age in the hierarchy of reasons that determine the interclonal and general modification variability was studied. It was demonstrated that the detected deviations of phenotypical characters in transplantation progeny from the donor and recipient phenotype belong by their nature to the temporary modifications and by normal parthenocloning are not the case in the next generation. Field of application: genetics.