Medvid O. Variability of Arabidopsis thaliana (L.) Heynh. fruit and flower structure under the influence of the mutant alleles of CLV1 and AP1 genes

Object: the genetic system of control of Arabidopsis thaliana flower and fruit structures. Aim: to study the genetic mechanism of formation of of Arabidopsis thaliana flower and fruit structures by the effect of recessive mutant genes CLV1 and AP1. Methods: hybridological, morphological, RAPD-analysis, statistical. Novelty: the complex justifications of influence of mutant allele of genes CLV1 аnd AP1 on the flower and fruit structure were proved. Results: the pleiotropic effect of mutant alleles of the mapped CLV1 and AP1 genes on flower and fruit development has been studied. Under the influence of the clv1-1 recessive allele in line N 45 a fruit of three- or four-celled capsule was developed instead of a silique. The clv1 mutant gene caused significant increase, up to 30%, in number of seeds in a fruit in comparison with effect of the er-1 gene. An intensified effect of ap1-1 allele as a minus–allele on the formation of all floral organs has been found. The ap1-3 mutant allele decreased number of sepals (~ up to 40%), stamens (~ up to 16%) and resulted in a complete absence of petals. The heptamarker line which contains the ap1-1, bp-1, clv1-1, dis2-1, er-1, gl1-1, tfl1-2 alleles (Lug62) is convenient for genetic analysis of arabidopsis since. On the basis of RAPD analysis the difference in the genome organization of the A. thaliana lines which contain the recessive mapped genes er-1; clv1-1,er-1; ap1-1,er-1 and ap1-1,clv1-1,er-1 as well as their genetic similarity has been proved. Application stage: the catalogue of Arabidopsis thaliana genetic collection of Lugansk National Agricultural University was published. The toretical and practical attainments have been included in the respective parts of the general genetics of Lugansk National Agricultural University. Application area: institution of higher education of MES, Minagropolityky of Ukraine