Demchenko O. The conformity of chromosomal aberration formation in humans' lymphocytes at low dose gamma irradiation

The object of study. Quantitative and qualitative regularities of the formation of chromosome aberrations in somatic human cells under the influence of small doses of radiation. The aim of the study was to determine the quantitative and qualitative regularities in the formation of chromosome aberrations under the influence of small doses of the testing in vitro radiation lymphosytes of human taking into account his individual radiation sensitivity (IRS) and influence of radioprotective preparation (inosine). Methods. Cytogenetic and statistical methods. Theoretical and practical results. Possibility of an objective determination of laws governing the formation of chromosome aberrations in cultures of human blood lymphocytes under the influence of radiation was proved and a method for finding drugs that can minimize and prevent the negative effects of radiation by increasing the stability of the genome of cells was also developed. The practical significance of the results obtained. An approach to the construction of the SCC (standart calibration curves) of "dose-effect" based on the frequency of radiation-induced chromosome aberrations in peripheral blood lymphocytes of healthy persons based on their IRS and the model of spline regression proposed was developed. This approach to construction of the SCC of "dose-effect" should be used for biodosimetry in cases of radiological incidents and accidents. Using of the preparation "inosine" as antimutagen and radioprotector in case, when of human somatic cells are influenced by irradiation in the range of small doses was suggested and justified by experiments at the genetic level of human somatic cells.