Bolotna I. The Structural Transformations of Animal’s Liver under Hyperhydration Imbalance of Water-Salt Metabolism in the Organism (anatomical experimental research)

The investigation object: The influence of hyperhydrational disorders of water and salts metabolism of mild, moderate and severe degree of the liver of white experimental rats. The investigation goal: To study morphofunctional changes in the liver of the animals of various age groups in the conditions of hyperhydrational disorders of water-and-salts metabolism and give reasons for possible usage of mexidole to control the disturbances of the organism. Methods of research: 1) microscopic method combined with organic measurement – to study the hepatic structure on the organic level; histological and morphological - to study the hepatic structure on tissue level; electron microscopic – to study morphofunctional capability of cell structures; 2) chemical analytic test – to estimate the indexes of the chemical composition of the liver; 3) statistic – to establish the possible effect of the obtained findings and disperse analysis of the degree of hyper hydration and animals’ age on the quantitative indexes of the research. Theoretical and practical value of the obtained results: The researches allowed clarifying of particular pathogenetic chains of affecting destroy of hyper hydration on macro- and microstructure of the liver. The obtained results appeared to be morphological basis for the establishment of pathological changes in the internal organs, particular in the liver that may be used in diagnostics of internal diseases, surgery, endocrinology, pediatrics, obstetrics and gynecology etc. The study of the mechanism of hyperhydration effect provides the background for purposeful discovery of effective hepatoprotectors; in particular, mexidole is suggested as a remedy of choice to control hyperhydratational damage of the liver. Scientific novelty of the obtained results: It is for the first time that basic particularities of the structural rebuilding and disturbances of chemical composition of the liver of young, mature and old age rates under the influence of hyperhydration of mild, moderate and severe degree that take place in the changing of morph functional structure of hepatocytes and decrease of their metabolic activity were revealed on the experimental material. The dependence of structural changes on the degree of hyperhydration that is manifested by linear character was discovered for the first time. Changes on the organic, cellular and subcellular level in the liver of rats under the influence of moded hyperhydration were investigated. It was mexidole that was used for the first time to control structural and functional disorders of the liver which developed as a result of hyperhydration of the organism. The degree of implementation: The results of experimental research are implemented in the academic curriculum and scientific investigation at the Chairs of Human Anatomy of Lugansk and Bookovina State Medical Universities, Vinnitsa National Medical University named after N.J. Pirogov, Crimean State Medical University named after S.I. Georiyevsky, Ternopol State Medical University named after I.Y. Gorbachevsky, Ukrainian Medical Stomatological Academy, at the Chair oh Human Anatomy and Histology of Medical Faculty of Ughgorod National University and at the Chair of Histology, Cytology and Embriology of Ivan Franko State Medical University too.