The thesis paper researches the regularities of changes in the structural organization and vascular bed in the spleen and the mesenteric lymph nodes under the influence of monosodium glutamate exemplified by male and female rats, after its abolition, alongside the possibilities to correct those, using orlistat (xenical) and melatonin.
A comprehensive morphological study of the spleen and mesenteric lymph nodes of white rats (both male and female) of reproductive age subjected to long-term exposure to monosodium glutamate, after its ceasure and correction of the effective changes by orlistat and melatonin. The data on morphological changes in the structure of the spleen and mesenteric lymph nodes at different points (2, 4, 6 and 8 weeks; 4 and 8 weeks after ceasure of the preceding six- and eight-week-long exposure) as sodium glutamate was being injected orally with the triggered changes being corrected by orlistat and melatonin (after 2 and 4 weeks with a previous six-week effect, and after 4 weeks with a previous eight-week effect). It has been established that the first structural signs of exposure to monosodium glutamate appear after two weeks of the exposure.
New morphometric data on the analysis of structural components of the spleen and mesenteric lymph nodes, namely the relative area of the white pulp of the spleen, including lymphoid periarterial sheaths and lymphoid nodules (mantle and marginal zone, germinal center, periarterial zone); the relative area of the red pulp of the spleen; outer and inner diameter of the central artery of the spleen; the relative area of the cortical substance of the mesenteric lymph nodes, in particular the lymphoid nodule (germinal center and mantle zone), marginal lymphatic sinus, cortical intermediate lymphatic sinus and cortical area; the relative area of the medullary substance of the mesenteric lymph nodes, in particular the medullary cords and medullary intermediate lymphatic sinuses and the cortico- medullary index of the white rats of reproductive age (both male and female) in the dynamics of 8-week exposure to monosodium glutamate, 4 and 8 weeks after discontinuation with previous six- and eight-week exposure, after 2 and 4 weeks of orlistat and melatonin correction alongside a preceding six-week exposure to monosodium glutamate, and 4 weeks after orlistat and melatonin correction eight-week exposure to monosodium glutamate. It has been confirmed that prolonged exposure to monosodium glutamate leads to an experimental high-calorie diet, which ultimately ensures the development of obesity.
It was established that the effect of monosodium glutamate on the body for two or four weeks does not cause significant, profound changes, i.e. it is safe. Destructive-degenerative changes after 6 weeks of administration, which are only partially corrected, after 4 and 8 weeks after its abolition, and profound destructive-degenerative changes after 8 weeks of exposure to monosodium glutamate are described.
An connection has been established between the duration of exposure to monosodium glutamate and the possibility of applying orlistat (xenical) and melatonin for correcting changes in the structural organization of the spleen and mesenteric lymph nodes of male and female white rats. After eight weeks of exposure to monosodium glutamate develop profound destructive-degenerative changes in structural components, which are only partially corrected, in contrast to the duration of six weeks exposure period.
