Poletay V. Influence of herbicides on the interstitial exchange of the carp's bile components.

Object: detoxification, excretory and metabolic functions of the liver. Aim: to study the effect of herbicides on intermediate exchange components of bile in carp of all ages. Methods: physiological, thin layer chromatography, direct densitometry, statistical. Results under the influence of herbicides in carp of all ages changed the ratio of individual fractions of bile acids, lipid fractions and pigments. Detected the development of adaptive processes in the carp of all ages under the influence herbtsydiv, which manifested itself in changing the activity of biosynthetic, detoxification and other processes in hepatocytes. Novelty: first conducted a comprehensive comparative study of flow processes in the individual intermediate links of the exchange of free and conjugated bile acids, the major fractions of lipids and bile pigments by the action on the body of carp one-and two-year age herbicides of different chemical structure and physico-chemical properties. First to show the selective effect zenkoru raundapu and the individual level of biosynthesis, conjugation, hydroxylation and translocation of certain fractions of bile acids through canalikular and sinusoidal membrane of hepatocytes, a comparative analysis of the impact zenkor and roundup the content and distribution of phospholipids, cholesterol, free fatty acids and triglycerides in liver tissue and biological fluids. Developed and patented a method of chromatographic separation of bile pigments, first discovered some features of the impact of herbicides on the ratio of free and conjugated derivatives biliverdynu and bilirubin in the tissues of fish. Area of application: physiology, biochemistry, hydrobiology, ecology.