The thesis is devoted to the influence of the chelates complex of Zinc and Copper with retinol acetate and ?-tocopherol on the indexes of immune reactivity in calves, vaccinated with salmonella vaccine. Based on the retrospective analysis the morbidity of calves with salmonellosis in the period between 1999 and 2009 was studied; Salmonella serotypes, obtained from pathological material from calves, beef and vegetal forage were determined. The results of the analysis have shown that the following salmonella serotypes from the material from cadavers of calves were obtained: Salm. dublin - from 149 cultured (2001) to 33 (2009); Salm. enteritidis - from 68 (2001) to 1 (2008); Salm. typhimurium - from 49 (2003) to 5 (2008); apart from the main host-adapted serotypes, Salm. choleraesuis, Salm. heidelberg, Salm. hamburg etc. were founded. From the vegetal forage Salm. typhimurium, Salm. enteritidis, Salm. typhisuis, Salm. heidelberg, Salm. panama and other were obtained. In the beginning of the experiment the insufficient concentration of vitamins A and E in blood serum of calves was established - vitamin A - 7,7±0,32 mcg/100 ml, vitamin E - 0,10±0,02 mg/100 ml. After the parenteral administration of retinol acetate the vitamin A concentration rose to 24,8±1,49 mcg/100 ml; after the administration of ?-tocopherol the vitamin E concentration rose to 0,32±0,02 mg/100 ml. After the administration of the salmonella vaccine vitamin A and E values in blood serum had tendency to rising. The salmonella vaccine administered to the calves caused the increase of values of ?-globulin fraction of protein in blood serum, in leukogram - the increase of monocytes; in all test groups of calves credibly rose the absolute quantity of lymphocytes, T and B lymphocytes, titer of salmonella antibodies and values of opsonic-phagocytic reaction, serum bactericidal activity (SBA) and serum lysozyme activity (SLA). The concentration of microelements Zinc and Copper in serum of calves before the feeding of chelates was Zn - 74,2±6,88 mcg/100 ml; Cu - 68,3±4,71 mcg/100 ml. After feeding of chelates of Zinc with retinol acetate the concentration of Zinc rose to 138,1±7,35 mcg/100 ml, and with ?-tocopherol - to 109,3±3,89 mcg/100 ml; Copper with retinol acetate the concentration of Copper - to 111,4±1,59 mcg/100 ml, Copper with ?-tocopherol - to 105,5±3,93 mcg/100 ml, complex of chelates of Zinc and Copper with retinol acetate and ?-tocopherol to 133±4,97 and 90,9±2,76 mcg/100 ml respectively.After the salmonella vaccine administration the serum concentration of Zinc tended to increase and that of Copper to decrease; in the group of animals that was immunized while administered chelates of Zinc and Copper with retinol acetate and ?-tocopherol they tended to increase. The values of immune reactivity to the salmonella vaccine in caves of test groups have credibly risen: ?-globulin fraction of protein, absolute quantity of lymphocytes, T and B lymphocytes, antibody titer, opsonic-phagocytic reaction, SBA and SLA. Based of the analysis of the results the methodic recommendations for the use of chelates of Zinc and Copper with retinol acetate and ?-tocopherol for the enhancement of immune reactivity in calves during the vaccination against salmonellosis were developed
