Kirpicheva I. Genetic control of morphological features of Arabidopsis thaliana (L.) Heynh. leaves.

While carrying out research on the topic of the thesis, which explores the genetic control of morphological features of leaves of Arabidopsis under the influence of mutant alleles of the mapped genes AN1, AN3, AS1, AS2, CP3, GL1, DIS2, their pleiotropic effects on several indicators of the leaves were proved. The effect of the an1-1 mutation reveals in increasing the stem length, reducing the width and length of the leaf blade. As a result, rosette and stem leaves of the Lug11 line acquire elliptical shape. The influence of the an3-1 mutant allele led to the development of elongated and back-lanceolate form of rosette leaves as a result of reducing the width of the leaf blades. When combining in a single genotype the two "minus-alleles" an1-1 and an3-1 according to the "width of the blade" trait, their independent effect on the shape of the leaves was defined. For the first time it was determined that mutations in the asymmetric leaves1 and asymmetric leaves2 genes determine the formation of leaf blades of various shapes (elliptical, circular, broadly ovate, ovate) at the same plant, as well as their partition (in blades, lobes, segments). It was determined that the ср3 gene determines the rounded form of a sheet by reducing the length of the leaf blade. When combining the as1-1 and ср3-1 minus-alleles a threerecessive line was obtained with an untypical for the A. thaliana specie leaf form, in which the width of the blade was more than its length. According to the nature of pubescence of the stem, rosette and stem leaves a complex differential display of the gl1-1 and dis2-1 genes action was revealed, when combining them in the same genotype. New transgenic lines were received and a positive effect of the genetic construction using the gene of resistance to phosphinothricin (bar) on the length of the leaf blade was determined.