Dotsenko R. Toxicological characteristics of Talstar and method for determination of its reactant in the objects of animal origin.

Thesis deals with the toxicological characteristics of Talstar 10 % e. c. (bifenthrin) on laboratory animals and egg chickens and the development of methods of bifentrin identification in feeds and animal facilities by means of gas-liquid chromatography. The developed method is based on removing insecticide by acetone from the examined sample, precipitation of coextractive substances from water-acetone solution in freezing chamber of a refrigerator, the redistribution of the preparation to hexane, extract purification by column chromatography with aluminum oxide, identification of the active substance by means of gas-liquid chromatography. A study of validation characteristics of the developed method has been carried out. Positive results of the research assure that the method will give accurate results when using it in practice by specialists of toxicological departments. In the acute experiments on white rats there was determined that, under hygienic classification of pesticides by L. I. Medved, Talstar 10 % e. c. (bifenthrin) should be attributed to strong substances (LD50 - 18,36 mg/kg). In experiments on chickens it has been determined that Talstar 10 % e. c. (bifenthrin) at doses of 3 and 9 mg/kg of body weight affects the carbohydrate metabolism and causes hypoglycemia, which begins on the first day and shows toxic lesion of liver, disorder of oxidation-recovery processes at metabolism of carbohydrates. Authentic increase in activity of AspAT, ALAT, LDH in the blood is a pathognomonic indicator of cytological symptoms of liver damage. Increased activity of ATPase indicates the beginning of recovery of physiological functions of the liver and other organs. Changes in iron, zinc and copper content show toxic effect of pyrethroids on mineral metabolism. The research on the allocation of bifenthrin with eggs indicate that even in a single receipt of pyrethroids to the body of birds, it is extracted with eggs every day for approximately a week, and it is extracted only with yolk.